Our platform's siRNA Designer Agent is a powerful tool that allows users to design small interfering RNAs (siRNAs) for any given nucleotide sequence or gene, across any genome. This agent streamlines the process of creating effective siRNA sequences for gene silencing experiments by providing flexible input and detailed output options tailored to your research needs.

Input Options:

• Gene Name or ID: Input the gene name (e.g., "BRCA1") or corresponding IDs (e.g., Ensembl ID "ENSG00000012048" or NCBI Gene ID "672") to automatically fetch the gene's sequence.

• Custom Nucleotide Sequence: Provide your own sequence if you want to target a specific region or gene variant.

• Organism/Genome: Select the organism or genome of interest, whether it's Homo sapiens, Mus musculus, or Saccharomyces cerevisiae.

Output Options:

• Optimal siRNA Sequences: The siRNA Designer will generate a list of potential siRNA sequences optimized for efficiency, off-target minimization, and specificity for the target gene or sequence.

• Thermodynamic Properties: The tool provides information on the thermodynamic stability of the designed siRNA, including GC content and melting temperature, to ensure optimal functionality.

• Targeting Information: The agent will specify the exact location on the gene or sequence where each siRNA binds, helping you target the most effective region for gene knockdown.

• Off-Target Analysis: Each siRNA is assessed for potential off-target effects by scanning similar sequences in the selected genome, ensuring specificity.

• Secondary Structure Avoidance: siRNA sequences are optimized to avoid regions of the gene with complex secondary structures (e.g., hairpins) that could impair efficacy.

This siRNA Designer Agent provides researchers with a reliable and efficient solution to generate highly targeted siRNAs, reducing experimental trial and error. Whether you're working on gene function studies or therapeutic applications, this tool ensures precision and effectiveness in your siRNA design.