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AI-agents

Within the chat, every interaction is managed by an AI agent. This section provides a brief description of each high-level AI agent.

Bio Expert

The Bio Expert agent assists with any biological inquiry, from offering detailed information and resources to providing relevant scientific articles. It can help design protocols, optimize experimental workflows, and suggest best practices based on the latest scientific advancements. All responses are text-based, generated from extensive training data. This agent can automatically browse the internet for additional up-to-date information or perform searches upon request, ensuring answers are accurate and comprehensive.

Sequence Fetcher

The Sequence Fetcher agent retrieves nucleotide or protein sequences, such as gene or transcript sequences. It accepts inputs like gene names (e.g., AQP1), gene IDs, transcript IDs, or other sequence identifiers. The agent can quickly fetch sequences from various databases, streamlining research and ensuring that users have accurate, up-to-date sequence information for their experiments.

Genome Fetcher

The Genome Fetcher agent provides comprehensive genomic information for any organism. It can retrieve full genomic sequences, coding DNA sequences (CDS), RNA sequences, protein sequences, and genetic annotations based on a user’s request. The agent accepts a variety of inputs, such as genome assembly accessions (e.g., GCA or GCF), common organism names (e.g., “mouse”), Latin names (e.g., Mus musculus), and even specific strains (e.g., “C57BL/6”). This agent ensures users have access to detailed genomic data tailored to their needs.

Sequence Editor

The Sequence Editor agent allows users to edit DNA or RNA sequences with precision. Whether inserting, deleting, or modifying specific regions of a sequence, it supports formats like FASTA and GenBank. The AI intelligently converts between formats when necessary, streamlining sequence editing and ensuring compatibility with downstream applications. This agent is ideal for tasks like optimizing constructs for cloning, expression, or other molecular biology workflows.

Oligo Designer

The Oligo Designer agent specializes in designing oligonucleotides, including probes, primers, and other short sequences. It considers user-defined properties such as melting temperature, GC content, and specificity. Results are presented with advanced visualization and in-depth analytics to help users optimize their oligo designs for PCR, qPCR, hybridization assays, or other applications requiring precise oligonucleotide synthesis.

CRISPR Guide Designer

This agent designs guide RNAs (gRNAs) for a variety of CRISPR-based genome editing technologies. It supports Cas9, Cas12, and Cas13 systems and their various applications, including nickase, base editing, and prime editing. The agent can design gRNAs for any species and incorporates flexible input criteria. It offers advanced visualizations and sophisticated analytics for each gRNA, helping users evaluate on-target and off-target effects, specificity, and overall editing efficiency.

siRNA Designer

The siRNA Designer agent is tailored for the design of small interfering RNAs (siRNAs), used in gene silencing experiments. It generates siRNAs with optimal properties for effective knockdown, including strand specificity and target accessibility. Outputs are accompanied by advanced visualization tools and analytics to aid in selecting the most effective siRNA for experimental needs, with considerations for minimizing off-target effects.

BLAST Agent

The BLAST Agent conducts sequence searches and analyses using the Basic Local Alignment Search Tool (BLAST). It accepts a wide range of input sequences, including DNA, RNA, or protein sequences, and compares them against vast biological databases. The agent provides detailed reports on sequence similarity, evolutionary relationships, and functional annotations, helping users identify homologous sequences, conserved domains, or potential functions of novel sequences.